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DNA fiber assay To investigate how the DNA-binding affinity of PDS5B affects replication fork protection, plasmids expressing PDS5B (WT) or PDS5B (17 A), as well as control or PDS5B-targeting siRNAs, were transfected using FuGENE HD (Promega) and Lipofectamine RNAiMAX (Invitrogen), respectively, according to the manufacturers’ instructions